#Dynamic light scatter full
#Dynamic light scatter software

Some of the high-throughput screening tasks accomplished with the DynaPro Plate Reader include: High-throughput, automated measurements performed directly in standard 96-, 384- or 1536-well microwell plates reveal new horizons for dynamic light scattering. The DLS Theory page contains additional details on the technique, including a video of Brownian motion. k D and A 2: input sample concentration.Particle concentration: input sample refractive index.Temperature is controlled and continuously measured in the instrumentįor advanced measurements, a few more parameters need to be selected or entered into DYNAMICS:.No sample-specific properties such as concentration or composition are required to assess size, size distributions and aggregation.

#Dynamic light scatter software

Moreover, DYNAMICS ® software contains an extensive, built-in solvent library with the most common solvents and buffer systems, including the option to create custom solvents. Solvent viscosity and refractive index are generally well-known and tabulated.The only necessary input parameters are solution viscosity and refractive index.ĭLS is a technique that can be readily applied for sizing unknown samples: The resulting intensity fluctuations in the light scattering detector are analyzed to determine a translational diffusion coefficient, which is converted to hydrodynamic radius via the Stokes-Einstein relationship (see sidebar). The sample is illuminated with a laser beam, and light waves scattered from the particles interfere constructively and destructively as the particles diffuse randomly. Pre-screening: Ideal to check sample quality before running other expensive and time-consuming biophysical methods such as TEM, AUC, SAXS/SANS, FFF and SEC.įor in-depth characterization of high-resolution size distributions and other physical properties, SEC-MALS and FFF-MALS offer complementary and orthogonal techniques to DLS.ĭLS determines the hydrodynamic size of a nanoparticle in solution by measuring the time scale of Brownian motion.Advanced properties: Measure particle concentration, nonspecific interactions ( A 2 or B 22) and differentiate between conformational changes and aggregation with the static light scattering option in the NanoStar and Plate Reader.Versatility: Beyond size and size distributions, measure other properties such as stability, stress-induced aggregation and molecular interactions.No shearing or filtering: always measure what’s in your solution.Low sample volume: Only 2 µL in the DynaPro NanoStar using quartz cuvettes, 4 µL in disposable cuvettes or standard microwell plates in the DynaPro Plate Reader.Non-destructive and fully recoverable: Samples may be transferred to other assays.

#Dynamic light scatter full

Full nanometer size range: from 0.5 nm to 2.5 µm in a single measurement, with a resolution up to a factor of 3-5 in size.

Dynamic & Electrophoretic Light ScatteringĭLS is an ideal screening tool for biopharmaceutical formulations and other nanomaterials.

hard sphere, globular, dendrimer, chain stiffness, and degree of branching). Hydrodynamic sizes are more easily measured than radii of gyration and can be measured over a wider range of sizes. The conversion from hydrodynamic radius to radius of gyration is a function of chain architecture (including questions of random coil vs. The hydrodynamic radius is not the same as the radius of gyration. Radius calculations are the same except for a factor of two.Īlso, a note to those interested in polymer size. That is, the determined particle size is the size of a sphere that diffuses the way as your particle.įor those who work with protein sizing and other areas where hydrodynamic radius is more commonly used, note that the development here is around diameter. Finally, and most importantly, it reminds the analyst that the particle size determined by dynamic light scattering is the hydrodynamic size. Temperature is even more important due to the viscosity term since viscosity is a stiff function of temperature. The first is that sample temperature is important, at it appears directly in the equation. However, the equation does serve as important reminder about a few points. The calculations are handled by instrument software.

T is thermodynamic temperature (we control this).

k B is Boltzmann’s constant (we know this).

D t is the translational diffusion coefficient (we find this by dynamic light scattering).D h is the hydrodynamic diameter (this is the goal: particle size!).